Vaccination Liberation - Information | |||
Legal: | Science: | Misc: | Searches: |
---|---|---|---|
Exemptions State_Chapter/ Resource_Contacts Avoid_Vaccinations Activism LegalNews |
Introduction Basic_Facts Package Inserts Ingredients of Vaccines Q_and_A Artificially Sweetened Times |
Membership Books Videos Tapes 100+ Anti-Vax links Vax_Cartoons Breaking News Planned_Events |
KeyWord_Index Index/Link_Pages Search_Our_Site Home_Page Index_Page Smallpox Alert! |
PCR testing
No More “Wild Measles,” Only Vaccine Genotypes?
May 9, 2019 By Catherine J. FrompovichWhat’s all the fuss about measles when there are definitive scientific mechanisms to ascertain which measles strains are involved, plus from where they originate, e.g., vaccines! How come the U.S. CDC and FDA are not adverse to using PCR (polymerase chain reaction) identification in order to “cherry pick” specific data regarding propagandized measles infections information, but will not permit PCR, as I understand, to be used by the medical profession to identify prophylactically, those infants and/or individuals who may present certain mitochondrial proclivities for vaccine adverse reactions, which, unequivocally, would disqualify them as vaccine candidates? Read rest of original story here:
File# 8148.php
PCR and Pertussis
Excerpts:Journal of Clinical Microbiology, August 2002, p. 2801-2805, Vol. 40, No. 8 Issues Associated with and Recommendations for Using PCR To Detect Outbreaks of Pertussis -- Lievano et al. 40 (8): 2801 -- J.. DISCUSSION The results of epidemiological and laboratory studies of these two outbreaks of cough illness illustrate the potential problems and consequences of relying exclusively on a PCR test to confirm pertussis. We confirmed by culture that pertussis was circulating in the community during these outbreaks. However, a significant epidemiological feature of one of these outbreaks was that unvaccinated children exposed to PCR-confirmed cases in day care centers did not become ill, whereas fully vaccinated children did. Thus, B. pertussis may not have been the primary or only cause of these outbreaks. Moreover, a positive PCR test due to transient colonization with the agent (15, 18, 20, 21, 22, 27, 28) or a falsely positive test could lead to misdiagnosis of pertussis in persons with cough illness due to other etiologies. Indeed, our results suggested that diagnostic reliance on a PCR assay for B. pertussis DNA together with probable false-positive test results likely overestimated the role of pertussis in these outbreaks. The PCR for B. pertussis DNA was used by a private laboratory to confirm the majority of cases in these two outbreaks (93% in outbreak 1 and 83% in outbreak 2). When compared with testing at the state reference laboratory, the majority of the B. pertussis PCR results from the private laboratory were not substantiated (Table 2). Moreover, during proficiency testing of 10 blinded specimens, the private laboratory reported false-positive results for one-third of the non-B. pertussis specimens. After changing primers towards the end of the second outbreak, the private laboratory retested the 10 samples and correctly identified all 10 specimens. This suggested that DNA contamination may have caused an uncertain number of false-positive results during the two outbreaks. PCR false-positive results can be due to DNA contamination, which may have been exacerbated by the larger-than-normal volume of samples processed during the outbreaks. The overdiagnosis of pertussis during these outbreaks had important public health consequences. These included the unnecessary treatment of asymptomatic individuals with a positive PCR result for B. pertussis DNA who may not have harbored B. pertussis, as well as inappropriate chemoprophylaxis for their close contacts. In outbreak 2, more than 1,100 persons in close contact with an individual with a positive PCR result for B. pertussis DNA were advised to take a 2-week course of chemoprophylaxis with a macrolide antibiotic. Staff at doctor offices, hospitals, and county health departments were overwhelmed with testing demands and follow-up of individuals who tested PCR positive for B. pertussis DNA. During outbreak 2, 10 percent of the 500-member staff of a community hospital, many of whom were asymptomatic, were furloughed for 5 days as a result of positive PCR tests. ... ... Read rest of article here: http://jcm.asm.org/cgi/content/full/40/8/2801 Issues Associated with and Recommendations for Using PCR To Detect Outbreaks of Pertussis -- Lievano et al. 40 (8): 2801 -- J.. |
|
a PCR for B. pertussis DNA was performed by the private laboratory and NYSDOH/WC pertussis laboratory on dual samples from the same patients.
b Specimens were prepared by NYSHD/WC Proficiency Testing Laboratory (four with B. pertussis) and blind PCR tested by the private laboratory and NYSDH/WC pertussis laboratory.
c n, no. of samples studied.